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Arabidopsis 2010 Project: Functional analysis of the Arabidopsis CLE
family of putative polypeptide signaling molecules
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| Jennifer C. Fletcher, University of California Berkeley, PI |
| Lewis J. Feldman, University of California Berkeley, Co-PI |
| Elliot M. Meyerowitz, California Institute of Technology, Co-PI |
The goal of this project is to determine the biological functions of the Arabidopsis CLE family of putative polypeptide signaling molecules. Intercellular signaling is essential for many plant processes, and 10% of predicted Arabidopsis proteins are classified as playing roles in signal transduction. Hundreds of putative receptors have been identified, but few extracellular signaling molecules are known. CLE proteins share sequence similarity with CLV3, a polypeptide ligand that binds the CLV1 receptor kinase to communicate cell fate information in meristems.
| The objectives of this proposal are: |
| Objective 1:analyze the mRNA expression pattern of each CLE gene during development and to determine the subcellular localization of the each protein. |
| Objective 2:determine the biological functions of each member of the CLE family, using loss-of-function and gain-of-function lines. |
| Objective 3:begin to identify the cognate receptors for the CLE polypeptides using genetic techniques. |
| Studies in progress: |
| Objective 1:promoter::GUS analysis; RNA in situ hybridization |
| Objective 2:cle T-DNA insertion line analysis; antisense and RNAi line analysis; 35S::CLE line analysis |
| Objective 3:CLE gene over-expression in LRR receptor kinase mutant backgrounds |
| Personnel: |
| Dr. Ji Hyung Jun, postdoc in the Fletcher lab |
| Elisa Fiume, graduate student in the Fletcher lab |
| Ling Meng, graduate student in the Feldman lab |
| Dr. Adrienne Roeder, postdoc in the Meyerowitz lab |
| Funding: |
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