| EDUCATION | | |
| 1974-1978 | A.B. in Genetics, University of California, Berkeley, CA, Undergraduate Research with Richard Calendar and Z. Renee Sung |
| 1978-1983 | Ph.D. in Cellular Developmental Biology, Harvard University, Cambridge, MA, Graduate Research with Frederick Ausubel |
| RESEARCH EXPERIENCE | | |
| 1983 | Postdoctoral Fellow in Genetics, Massachusetts General Hospital, Boston, MA, Laboratory of Frederick Ausubel |
| 1984 | Academia Sinica Visiting Fellow, Shanghai Institute of Plant Physiology, Shanghai, China, Laboratory of San-Chiun Shen |
| 1984-1986 | National Science Foundation Postdoctoral Fellow, University of California, San Diego, CA, Laboratory of Stephen H. Howell |
| 1986-present | Senior Scientist, Plant Gene Expression Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, CA 94710. Tel: (510) 559-5909, Fax: (510) 559-5678, Email: ow@pgec.ars.usda.gov |
| PUBLICATIONS | | |
| A. | Refereed Journal Articles |
| A1. | 1980 | Kahn, M., Ow, D., Sauer, B., Rabinowitz, A., Calendar, R. Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids. Molecular & General Genetics 177: 399-412 |
| A2. | 1980 | Ow, D.W., Ausubel, F.M. Recombinant P4 bacteriophages propagate as viable lytic phages or as autonomous plasmids in Klebsiella pneumoniae. Molecular & General Genetics 180: 165-175 |
| A3. | 1981 | Sauer, B., Ow, D., Ling, L., Calendar, R. Mutants of satellite bacteriophage P4 that are defective in the suppression of transcriptional polarity. Journal of Molecular Biology 145: 29-46 |
| A4. | 1983 | Ow, D.W., Ausubel, F.M. Regulation of nitrogen metabolism genes by the nifA gene product in Klebsiella pneumoniae. Nature (London) 301: 307-313 |
| A5. | 1983 | Sundaresan, V., Jones, J.D.G., Ow, D.W., Ausubel, F.M. Klebsiella pneumoniae nifA product activates the Rhizobium meliloti nitrogenase promoter. Nature (London) 301: 728-732 |
| A6. | 1983 | Ow, D.W., Sundaresan, V., Rothstein, D.M., Brown, S.E., Ausubel, F.M. Promoters regulated by the glnG (ntrC) and nifA gene products share a heptameric consensus sequence in the -15 region. Proceedings of the National Academy of Sciences USA 80: 2524-2528 |
| A7. | 1983 | Sundaresan, V., Ow, D.W., Ausubel, F.M. Activation of Klebsiella pneumoniae and Rhizobium meliloti nitrogenase promoters by gln (ntr) regulatory proteins. Proceedings of the National Academy of Sciences USA 80: 4030-4034. |
| A8. | 1983 | Ow, D.W., Ausubel, F.M. Conditionally replicating plasmid vectors that can integrate into the Klebsiella pneumoniae chromosome via bacteriophage P4 site-specific recombination. Journal of Bacteriology 155: 704-713. |
| A9. | 1985 | Ow, D.W., Xiong, Y., Gu, Q., Shen, S.C. Mutational analysis of the Klebsiella pneumoniae nitrogenase promoter: sequences essential for positive control by nifA and ntrC (glnG) products. Journal of Bacteriology 161: 868-874 |
| A10. | 1986 | Ow, D.W., Wood, K.V., DeLuca, M., de Wet, J.R., Helinski, D.R., Howell, S.H. Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856-859. |
| A11. | 1987 | Ow, D.W., Jacobs, J., Howell, S.H. Functional regions of the CaMV 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity. Proceedings of the National Academy of Sciences USA 84: 4870-4874. |
| A12. | 1990 | Schneider, M., Ow, D.W., Howell, S.H. The in vivo pattern of firefly luciferase expression in transgenic plants. Plant Molecular Biology 14: 935-947 |
| A13. | 1990 | Joshi, R.L., Joshi, V., Ow, D.W. BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells. EMBO (European Molecular Biology Organization) Journal 9: 2663-2669 |
| A14. | 1990 | Dale, E.C., Ow, D.W. Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91: 79-85 |
| A15. | 1991 | Dale, E.C., Ow, D.W. Gene transfer with subsequent removal of the selection gene from the host genome. Proceedings of the National Academy of Sciences USA 88: 10558-10562 |
| A16. | 1992 | Bayley, C.C., Morgan, M., Dale, E.C., Ow, D.W. Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Molecular Biology 18: 353-362 |
| A17. | 1992 | Abrahamson, S.L., Speiser, D.M., Ow, D.W. A gel electrophoresis assay for phytochelatins. Analytical Biochemistry 200: 239-243 |
| A18. | 1992 | Bayley, C., Trolinder, N., Ray, C., Morgan, M., Quisenberry, J.E., Ow, D.W. Engineering 2,4-D resistance into Cotton. Theoretical & Applied Genetics 83: 645-649. |
| A19. | 1992 | Speiser, D.M., Abrahamson, S.L., Banuelos, G., Ow, D.W. Brassica juncea produces a phytochelatin-cadmium-sulfide complex. Plant Physiology 99: 817-821. |
| A20. | 1992 | Ortiz, D.F., Kreppel, L., Speiser, D.M., Scheel, G., McDonald, G., Ow, D.W. Heavy metal tolerance in the fission yeast requires an ATP binding cassette-type vacuolar membrane transporter. EMBO (European Molecular Biology Organization) Journal 11: 3491-3499 |
| A21. | 1992 | Speiser, D.M., Ortiz, D.F., Kreppel, L., Scheel, G., McDonald, G., Ow, D.W. Purine biosynthetic genes are required for cadmium tolerance in Schizosaccharomyces pombe. Molecular and Cellular Biology 12:5301-5310 |
| A22. | 1993 | Juang, R.-H., McCue, K.F., Ow, D.W. Two purine biosynthetic enzymes that are required for cadmium tolerance in Schizosaccharomyces pombe utilize cysteine sulfinate in vitro. Archives of Biochemistry & Biophysics 304: 392-401 |
| A23. | 1993 | van Haaren, M.J.J., Ow, D.W. Prospects of applying a combination of DNA transposition and site-specific recombination in plants. Plant Molecular Biology 23: 525-533 |
| A24. | 1993 | Ow, D.W. Phytochelatin-mediated cadmium tolerance in Schizosaccharomyces pombe. In Vitro Cellular Developmental Biology 29P: 213-219. |
| A25. | 1994 | Qin, M., Bayley, C., Stockton, T., Ow, D.W. Cre recombinase mediated site-specific recombination between plant chromosomes. Proceedings of the National Academy of Sciences USA 91: 1706-1710 |
| A26. | 1995 | Ow, D.W., Medberry, S.L. Genome manipulation through site-specific recombination. Critical Reviews in Plant Sciences 14: 239-261. |
| A27. | 1995 | Medberry, S.L., Dale, E., Qin, M., Ow, D.W. Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination. Nucleic Acids Research 23: 485-490 |
| A28. | 1995 | Ortiz, D.F., Ruscitti, T., McCue, K., Ow, D.W. Transport of metal-binding peptides by HMT1, a fission yeast ABC-type vacuolar membrane protein. Journal of Biological Chemistry 270: 4721-4728 |
| A29. | 1995 | Albert, H., Dale, E.C., Lee, E., Ow, D.W. Site-specific integration of DNA into wild type and mutant lox sites placed in the plant genome. The Plant Journal 7: 649-659 |
| A30. | 1995 | Qin, M., Lee, E., Zankel, T., Ow, D.W. Site-specific cleavage of chromosomes in vitro through Cre-lox recombination. Nucleic Acids Research 23: 1923-1927 |
| A31. | 1996 | Cunningham, S., Ow, D.W. Promises and prospects of phytoremediation. Plant Physiology 110: 715-719. |
| A32. | 1996 | Ow, D.W. Recombinase-directed chromosome engineering in plants. Current Opinion in Biotechnology 7: 181-186 |
| A33. | 1996 | Ow, D.W. Heavy metal tolerance genes: prospective tools for bioremediation. Resources, Conservation and Recycle 18: 135-149 |
| A34. | 1997 | Perego, P., VandeWeghe, J., Ow, D.W., Howell, S.B. The role of determinants of cadmium sensitivity in the tolerance of Schizosaccharomyces pombe to cisplatin. Molecular Pharmacology 51: 12-18 |
| A35. | 1999 | VandeWeghe, J., Ow, D.W. A fission yeast gene for mitochondrial sulfide oxidation. Journal of Biological Chemistry 274: 13250-13257 |
| A36. | 1999 | Srivastava, V., Anderson, O.D., Ow, D.W. Single-copy transgenic wheat generated through the resolution of complex integration patterns. Proceedings of the National Academy of Sciences USA 96: 11117-11121 |
| A37. | 2000 | Choi, S., Begum, D., Koshinsky, H., Ow, D.W., Wing, R.A. A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination. Nucleic Acids Research 28: e19, i-vii. |
| A38. | 2000 | Koshinsky, H.A., Lee, E., Ow, D.W. Cre-lox site-specific recombination between Arabidopsis and tobacco chromosomes. Plant Journal 23: 715-722 |
| A39. | 2000 | Day, C.D., Lee E., Kobayashi, J., Holappa, L.D., Albert, H., Ow, D.W. Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced, Genes & Development 14: 2869-2880 |
| A40. | 2001 | Ow, D.W. The right chemistry for marker gene removal? Nature Biotechnology 19: 115-116 |
| A41. | 2001 | Thomason, L.C., Calendar, R., Ow, D.W. Gene insertion and replacement in Schizosacchromyces pombe mediated by the Streptomyces bacteriophage phiC31 site-specific recombination system, Molecular Genetics & Genomics 265: 1031-1038 |
| A42. | 2001 | Srivastava, V., Ow D.W. Single copy primary transformants of maize obtained through the co-introduction of a recombinase-expressing construct, Plant Molecular Biology 46 :561-566 |
| A43. | 2002 | Vande Weghe, J., Ow, D.W. 2001. Accumulation of metal-binding peptides in fission yeast requires hmt2. Molecular Microbiology 42: 29-36 |
| A44. | 2002 | Ow, D.W. 2002. Recombinase-directed plant transformation for the post genomic era. Plant Molecular Biology 48: 183-200 |
| A45. | 2002 | Srivastava, V., Ow D.W. Biolistic mediated site-specific integration in rice. Molecular Breeding 8: 345-350. |
| A46. | 2003 | Belteki, G., Gertsenstein, M., Ow, D.W., Nagy, A. Site-specific cassette exchange and germline transmission with mouse ES cells expressing fC31 integrase. Nature Biotechnology 21: 321-324. |
| A47. | 2003 | Srivastava, V., Ow, D.W. Rare instances of Cre-mediated deletion product maintained in transgenic wheat. Plant Molecular Biology 52: 661-668. |
| B. | Book Chapters & Symposium Proceedings |
| B1. | 1980 | Ausubel, F.M., Janssen, K.A., Ow, D.W., Riedel, G.E., Ruvkun, G.B., Cannon, F.C., and Cannon, M.C. The use of recombinant DNA techniques in the study of nitrogen-fixing genes. In: Nitrogen Fixation (Steward, W.D.P., Gallon, J.R., eds.) Academic Press, London, pp. 395-419. |
| B2. | 1982 | Ausubel, F.M., Brown, S.E., deBruijn, F.J., Ow, D.W., Riedel, G.E., Ruvkun, G.B., and Sundaresan, V. Molecular cloning of nitrogen fixation (nif) genes from Klebsiella pneumoniae and Rhizobium meliloti. In: Genetic Engineering (Setlow, J.K., Hollaender, A., eds.) Plenum Press, New York, pp. 169-198. |
| B3. | 1983 | Ausubel, F.M., Ow, D.W., Sundaresan, V. Conservation of the regulatory mechanisms regulating the expression of nitrogen fixation genes in Rhizobium meliloti and Klebsiella pneumoniae. In: Structure And Function Of Plant Genomes (Ciferri, O., Dure III, L., eds.) Plenum Press, New York, pp. 253-262. |
| B4. | 1983 | Sundaresan, V., Jones, J., Ow, D.W., Ausubel, F.M. Regulation of nif genes in Rhizobium meliloti and Klebsiella pneumoniae. In: Gene Expression, UCLA Symposium on Molecular and Cellular Biology, New Series, Vol. 8 (Hamer, D., Rosenberg, M., eds.) Alan R. Liss, Inc., New York, pp. 175-185. |
| B5. | 1983 | Zimmerman, L., Szeto, W., Ow, D.W., Ausubel, F.M. A region which regulates nitrogenase expression in Rhizobium meliloti. In: Plant Molecular Biology, UCLA Symposium on Molecular and Cellular Biology, New Series, Vol. 12 (Goldberg, R.B., ed.), Alan R. Liss, Inc., New York, pp. 237-242. |
| B6. | 1984 | deBruijn, F.J., Sundaresan, V., Szeto, W.W., Ow, D.W., Ausubel, F.M. Regulation of nitrogen fixation (nif) genes of Klebsiella pneumoniae and Rhizobium meliloti: role of nitrogen regulation (ntr) genes. In: Advances In Nitrogen Fixation Research (Veeger, C., Newton, W.E., eds.), Martinus Nijoff/Dr. W. Junk, Hague, pp. 627-633. |
| B7. | 1985 | Ow, D.W., Gu, Q., Xiong, Y., Zhu, J., Shen, S.C. Regulation of Klebsiella pneumoniae nitrogen fixation gene promoters by regulatory proteins ntrC, nifA, and nifL. In: Nitrogen Fixation Research Progress (Evans, H.J., Bottomley, P.J., Newton, W.E., eds.), Martinus Nijoff, Dordrecht, pp. 461-467. |
| B8. | 1989 | Ow, D.W., Howell, S.H. Incorporation of firefly luciferase gene into plant cells. In: Biotechnology In Agriculture And Forestry, Vol. 9 "Plant Protoplasts & Genetic Engineering II" (Bajaj, Y.P.S., ed.), Springer-Verlag, Heidelberg, pp. 376-387. |
| B9. | 1989 | Howell, S.H., Ow, D.W., Schneider, M. Use of the firefly luciferase gene as a reporter of gene expression in plants. In: Plant Molecular Biology Manual (Gelvin, S.B., Schilperoot, R.A., eds.), Kluwer Academic Press, pp. 1-11. |
| B10. | 1991 | Kahn, M.L., Ziermann, R., Deho, G., Ow, D.W., Sunshine, M.G., Calendar, R. Bacteriophage P2 and P4. In: Methods In Enzymology, vol 204, pp. 264-280, "Bacterial Genetic Systems" (Miller, J.H., ed.), Academic Press, pp. 264-280. |
| B11. | 1994 | Ow, D.W., Ortiz, D.F., Speiser, D.M., McCue, K.F. Molecular genetic analysis of cadmium tolerance in Schizosaccharomyces pombe. In: Metal Ions In Fungi (Winkelmann, G., Winge, D., eds.), Marcel Dekker, Inc., New York, pp. 339-359. |
| B12. | 1995 | Koshinsky, H.A., Liao, H.K., Ow, D.W. Progress in screening microorganisms for gossypol-degrading ability. In: Biochemistry Of Cotton (Proceedings of the Biochemistry of Cotton Workshop, Galveston, Texas, 09/28/94-09/30/94), Cotton Inc., pp. 19-22. |
| B13. | 1995 | Ow, D.W., Albert, H., Dale, E., Lee, E., Stockton, T. Cre-lox site-specific integration of DNA into the plant genome. In: Modification Of Gene Expression And Non-Mendelian Inheritance (Proceedings of the Japanese-American joint symposium, Tsukuba, Japan, 01/24/95-01/26/95) (Oono, K., Takaiwa, F., eds.), National Institute of Agrobiological Resources, Sato Press, Mito, pp. 225-234. |
| B14. | 1995 | Morgan, M.K., Ow, D.W. Polyethylene glycol-mediated transformation of tobacco leaf mesophyll protoplasts: an experiment in the study of Cre-lox recombination. In: Methods In Plant Molecular Biology, A Laboratory Manual (Maliga, P., Klessig, D., Cashmore, A., Gruissem W., Varner, J., eds.), Cold Spring Harbor Press, pp. 1-17. |
| B15. | 1997 | Ow, D.W. Marker-free transgenic plants. In: Proceedings Of The 2nd Central And Eastern European Conference For Regional And International Cooperation On Safety In Biotechnology. Federal Environmental Agency, Vienna, Austria, pp. 25-28. |
| B16. | 1997 | Ow, D.W. Techniques for creating marker free transgenic plants. In: Nordic Seminar on Antibiotic Resistance Marker Genes and Transgenic Plants. Norwegian Biotechnology Advisory Board, Oslo, Norway, pp. 59-68. |
| B17 | 1997 | Koshinsky, H, Ow, D.W. Recombinase systems in plants. In: Proceedings Of The 1997 Tappi (Technical Association Of The Pulp And Paper Industry) Biological Sciences Symposium (10/19/97-10/23/97, San Francisco), pp. 295-297. |
| B18. | 1998 | Ow, D.W., Quisenberry, J.E. Transgenic cotton resistant to 2,4-D. In: Biotechnology In Agriculture And Forestry, vol. 42, "Cotton" (Bajaj, Y.P.S., ed.), Springer-Verlag, Heidelberg, pp. 291-297. |
| B19. | 1998 | Albert, H., Ow, D.W. Site-specific recombination directed plant transformation. In: Somaclonal Variation And Induced Mutation In Crop Improvement (Jain, S.M., Ahloowalia, B.S., Brar, D.S., eds.), Kluwer Academic Press, The Netherlands, pp. 503-518. |
| B20. | 1998 | Ow, D.W. Prospects of engineering heavy metal detoxification genes in plants. In: Engineering Crops For Industrial Uses (Shewry, P., ed.), Portland Press, pp. 111-124. |
| B21 | 2000 | Ow, D. Marker Genes. In: Proceedings of the Joint FAO/WHO Expert Consultation on Foods Derived from Biotechnology (May 29-June 2, 2000, Geneva, Switzerland), pages 1-6. |
| B22 | 2000 | Ow, D.W., Kim, J.H., Fox, T., Sin, M. Cloning heavy metal tolerance genes. Papers of the Environmental Management Science Program Workshop, Atlanta, GA, April 25-27, 2000, pages 1-9. |
| B23 | 2000 | Ow, D.W. Transgenic Plants, Then and Now: A Personal Perspective. In: Proceedings of the 2000 Cotton Research Meeting and Summaries of Cotton Research in Progress (Oosterhuis, D.M., ed.), Univ of Arkansa Press, pp. 15-21. http://www.uark.edu/depts/agripub/Publications/specialreports/198ms4.pdf |
| B24. | 2000 | Albert, H., Ow, D.W. Cre-lox directed integration of transgenes into tobacco. In: Plant Molecular Biology Manual (Gelvin, S.B., Schilperoot, R.A., eds.), Kluwer Academic Press, Dordrecht. Pp. N3 1-13. |
| B25 | 2002 | Ow, D.W. Recombinasse-directed transgene placement. In: Proceedings of the 7th International Symposium on the Biosafety of Genetically Modified Organisms, Beijing, China, pp. 13-20. http://www.bba.de/gentech/isbgmo.pdf |
| B26 | 2003 | Ow, D.W. Site-specific gene stacking method. In: Plant Biotechnology 2002 and Beyond (Vasil, I.K., ed.), Kluwer Academic Press, Dordrecht, pp. 215-218. |
| B27 | 2003 | Ow, D.W. Genetically Modified Plants - Current Technology & Future Prospects. Commissioned review for the Pan American Health Organization /World Health Organization (PAHO/WHO), Food and Agriculture Organization (FAO), and Inter American Institute for Cooperation on Agriculture (IICA) Joint Meeting on Safety of Food Produced by Biotechnology, Buenos Aires, Argentina, February 20-11, 2003. http://www.panalimentos.org |
| C. | Published or Issued Patents |
| C1. | 1993 | Ow, D.W., Dale, E.C. "Selection-gene-free transgenic plants", International patent publication WO 93/01283, Jan. 21,1993. |
| C2. | 1996 | McElroy M.D., Helinski, D.R., Wood, K.V., DeWet, J.R., Ow, D.W., Howell, S.H. "Recombinant expression Of Coleoptera luciferase", U.S. Patent 5,583,024, Issued Dec. 10, 1996. |
| C3. | 1997 | McElroy M.D., Helinski, D.R., Wood, K.V., DeWet, J.R., Ow, D.W., Howell, S.H. "DNA sequences encoding coleoptera luciferase activity", U.S. Patent 5,674,713, Issued Oct. 7, 1997. |
| C4. | 1997 | McElroy M.D., Helinski, D.R., Wood, K.V., DeWet, J.R., Ow, D.W., Howell, S.H. "Recombinantly produced Coleoptera luciferase and fusion proteins thereof", U.S. Patent 5,700,673, Issued Dec. 23, 1997. |
| C5. | 2000 | Ow, D.W., Srivastava, V. "Resolution of complex integration patterns to obtain single copy transgenes", WO 99/23202, May 14, 1999; U.S. Patent No. 6,114,600. Issued Sept. 5, 2000. |
| C6. | 2001 | Ow, D.W., Calendar, R., Thomason, L. "DNA recombination in eucaryotic cells by the bacteriophage phiC31 recombination system", International (WIPO PCT) publication WO 01/07572, Feb.1, 2001; U.S. Patent 6,746,870, issued June 8, 2004. |
| D. | Thesis |
| D1. | 1983 | Ow, D.W. Control of nitrogen fixation genes by the Klebsiella pneumoniae nifA product. Ph.D. thesis, Harvard University. |
